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routineda: Development of an antibiotics screening assay – the sampling of hospital water

As part of the RoutinEDA project and as an expansion of the current Effect-directed Analysis platform, we are developing a bioassay that detects the presence of antibiotics in environmental matrices such as water and soil. Bacteria that have been made increasingly susceptible to antibiotics are exposed to concentrated environmental extracts. The inhibition of bacterial growth may indicate the presence of antibiotic-like compounds in the samples.   

In the first week of February 2019, we collected effluent water directly from a hospital in Arnhem. We will screen for antibiotics in the extracts that will be prepared from the samples, using the developed bioassay.

We thank Arnhem municipality for providing the opportunity to sample.

 

Project update: cost effective removal of Cecs

Our project 'Cost-Effective Removal of Contaminants of Emerging Concern' started in January, last year, when Ana Belén Ríos Miguel began her PhD at the department of Microbiology at the Radboud University (RU). A few months later, Tamara van Bergen started her PhD at the department of Environmental Science (RU). Together with all project supervisors, Rosalie van Zelm, Ad Ragas, Cornelia Welte, Mike Jetten and project leader Jan Hendriks, we really enjoy the synergistic cooperation of our departments in the CER-CEC project!

    

On the left picture Tamara van Bergen is measuring samples of a pilot experiment at the GC-MS, and on the right, Ana Rios is preparing nitrifying bioreactors. In the near future, we will grow our own CER-CEC microbial communities!

In October, we presented our first results to the stakeholders of our project. Several waterboards, drinking water companies, water technology companies, and governmental agencies are involved in our project. They were very enthusiastic in the first kick-off meeting and together we discussed the list of chemicals to be investigated in the project, and the selected of suitable wastewater treatment plants to be sampled. We presented our first objectives towards our shared goal: on the one hand assessing and prediction biodegradation of CECs (Environmental Science) and on the other hand optimizing CEC removal and working towards an application (Microbiology). The first results of a shared pilot experiment were presented, which will lead to an assessment of biodegradation rates of several chemicals of emerging concern. At the moment, the biodegradation assays still need optimization and a method for measuring our chemicals in wastewater samples needs to be developed. We will work on those topics the coming period and in addition, Ana Rios will also focus on starting up bioreactors and enrichment experiments. In the meantime, Tamara van Bergen will gain more knowledge on how biological mechanisms affect biodegradation of chemicals and will explore ways to include microbial community changes into biodegradation models. This is expected to lead to an increased understanding and prediction of biodegradation in wastewater treatment plants.

The Kick-off and QA day was a success

First CEC-Partnership programme day and quality assurance workshop

 Today we will have the first CEC-Partnership programme day and quality assurance workshop @ KWR Nieuwegein.

The programme of the day is:

10:45-11:00

Registration and coffee

11:00-11:20

Introduction about the CEC-partnership

Bas van der Wal – STOWA

11:20-11:40

Introduction TTW

Stefan Jongerius – NWO domain TTW

11:40 – 12:30

Introduction projects 1 and 2 (15 min + 5 min questions)

Project leaders

12:30-13:30

Lunch

13:30-14:30

Introduction project 3, 4, 5

Project leaders

14:30-14:45

Break

14:45-16:30

QA Workshop  
Annemieke Kolkman

16:30

Tour + drinks

 

The venue

KWR Watercycle Research Institute – Groningenhaven 7, Nieuwegein

CEC-partnership.nl is live!

CEC-partnership.nl is live!

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